This kit allows for the determination of CMV IgG expression in
Human serum, and other biological fluids.
Principle of the assay
The kit assay CMV IgG level in the sample, use Purified antigen to
coat microtiter plate wells, make solid-phase antigen, then add CMV
IgG to wells, Combined With CMV IgG, after washing and removing
non-combinative antibody and other components ,then Combined
antigen which with HRP labeled become
antigen–antibody-enzyme-antigen complex, after washing Completely,
Add TMB substrate solution,, TMB substrate becomes blue color At
HRP enzyme-catalyzed, reaction is terminated by the addition of a
sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm. Compared with the
CUTOFF value, according to this to judge CMV IgG exist in the
sample or not.